Magnetic Tweezers Measurement of Single Molecule Torque
نویسندگان
چکیده
منابع مشابه
Magnetic Tweezers for Single-Molecule Experiments
Over the last decade, single-molecule techniques have proven their wide applicability in the study of processive motor proteins and other enzymes, yielding insight into their kinetics and mechanochemistry. In the context of force spectroscopy of protein–nucleic acid interactions, optical tweezers, magnetic tweezers, and atomic force microscopy have made important contributions. Advantages of ma...
متن کاملMagnetic Tweezers for the Measurement of Twist and Torque
Single-molecule techniques make it possible to investigate the behavior of individual biological molecules in solution in real time. These techniques include so-called force spectroscopy approaches such as atomic force microscopy, optical tweezers, flow stretching, and magnetic tweezers. Amongst these approaches, magnetic tweezers have distinguished themselves by their ability to apply torque w...
متن کاملMultiplexed single-molecule measurements with magnetic tweezers.
We present a method for performing multiple single-molecule manipulation experiments in parallel with magnetic tweezers. We use a microscope with a low magnification, and thus a wide field of view, to visualize multiple DNA-tethered paramagnetic beads and apply an optimized image analysis routine to track the three-dimensional position of each bead simultaneously in real time. Force is applied ...
متن کاملTorque measurement at the single-molecule level.
Methods for exerting and measuring forces on single molecules have revolutionized the study of the physics of biology. However, it is often the case that biological processes involve rotation or torque generation, and these parameters have been more difficult to access experimentally. Recent advances in the single-molecule field have led to the development of techniques that add the capability ...
متن کاملSingle-molecule magnetic tweezers studies of type IB topoisomerases.
The past few years have seen the application of single-molecule force spectroscopy techniques to the study of topoisomerases. Magnetic tweezers are particularly suited to the study of topoisomerases due to their unique ability to exert precise and straightforward control of the supercoiled state of DNA. Here, we illustrate in a stepwise fashion how the dynamic properties of type IB topoisomeras...
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ژورنال
عنوان ژورنال: Nano Letters
سال: 2009
ISSN: 1530-6984,1530-6992
DOI: 10.1021/nl900631w